Alternative splicing in response to Listeria infection

A team from ENS Paris with the help from GenomiqueENS platform studied the response and the reorganization of gene expression in cells due to the introduction of bacteria.

They combined long- and short-read transcriptomic analyses of the response of intestinal epithelial cells to infection by the foodborne pathogen Listeria monocytogenes to gain isoform-level resolution of these modes of regulation.

Among the most striking isoform-based types of regulation, they discovered that expression of a stress response regulator and of several splicing factors switched from canonical transcripts to nonsense-mediated decay-sensitive isoforms by inclusion of ‘poison exons’.

Their findings are published in Nucleic Acids Research (Link to the paper)

Nucleic Acids Res, Volume 51, Issue 22, 11 December 2023, Pages 12459–12475, © The Author(s) 2023. Published by Oxford University Press on behalf of Nucleic Acids Research.