Introduction

The facility was founded in 1999 when the Montpellier – Languedoc-Roussillon Genopole was set up. It is located on the Arnaud de Villeneuve campus, within the Functional Genomics Institute.

This platform offers the following services

• High-throughput sequencing
• Bio-statistics and bioinformatics

Applications available include de novo sequencing or re-sequencing of genomes, the study of the transcriptome of numerous animal, plant and prokaryotic species, but also techniques for studying chromatin structure and dynamics (ChIP-seq, 4C, Hi-C, etc.) and epigenetic modifications (DNA methylation). Genomic approaches on single cells in suspension or tissue sections are currently being developed.

The plateform is run by a team of 10 engineers: 7 molecular biologists and 3 bioinformaticians.

Equipments

Development objectives

The platform is currently working on methods to limit sequencing costs and reduce the biases linked to library construction methods, particularly in the context of transcriptomic studies.
It is also working on a method for evaluating the stability of the genome of cell lines (particularly iPS) during the various passages necessary for their growth.

Main achievements

• de la Fuente C. et al. (2024) Glutaredoxin regulation of primary root growth is associated with early drought stress tolerance in pearl millet. Elife. 12:RP86169. doi: 10.7554/eLife.86169
• Salles J. et al. (2024) Differential DNA methylation in iPSC-derived dopaminergic neurons: a step forward on the role of SNORD116 microdeletion in the pathophysiology of addictive behavior in Prader-Willi syndrome. Mol Psychiatry. 29:2742-2752. doi: 10.1038/s41380-024-02542-4.
• Boulanger M. et al. (2023) DeSUMOylation of chromatin-bound proteins limits the rapid transcriptional reprogramming induced by daunorubicin in acute myeloid leukemias. Nucleic Acids Res. 51:8413-33. doi: 10.1093/nar/gkad581.
• Bouchereau W. et al. (2022) Major transcriptomic, epigenetic and metabolic changes underly the pluripotency
  continuum in rabbit preimplantation embryos. Development 19. doi: 10.1242/dev.200538.
• Geffroy B. et al. (2021) Unraveling the genotype by environment interaction in a thermosensitive fish with a
  polygenic sex determination system. PNAS U S A 118. doi: 10.1073/pnas.2112660118.
• Le Roy C. et al. (2021) Convergent morphology and divergent phenology promote the coexistence of Morpho
  butterfly species. Nat Commun. 12. doi: 10.1038/s41467-021-27549-1.
• Reynès C et al. (2020) ISoLDE: a data-driven statistical method for the inference of allelic imbalance in datasets with reciprocal crosses. Bioinformatics. 36(2):504-13. doi: 10.1093/bioinformatics/btz564
• Puighermanal E et al. (2020) Functional and molecular heterogeneity of D2R neurons along dorsal ventral axis in the striatum. Nat Commun. 11:1957. doi: 10.1038/s41467-020-15716-9.

Last update January 2025