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Sequencing Platform Genomic Paris Centre - Institut Curie

-Targeted resequencing and whole genome sequencing, ChIP-seq, RNA-seq, SmallRNA.

- Fragment libraries, paired-end and mate pair libraries

- Amplicon sequencing, target enrichment, exome and whole genome sequencing

- Identification of polymorphisms, mutations and genome rearrangements..

HiSeq 2000 (600Gb)
HiSeq 2500 (600Gb en High Output et 120Gb en Rapid Run)
2 x Ion Torrent PGM (10Mb-1Gb)
Ion Proton (10Gb)

Main achievements:
- BRCA1 and BRCA2 (targeted) : C. Houdayer, D. Stoppa-Lyonnet,

- BRCAX (exome) :M-H. Stern, D. Stoppa-Lyonnet

- HPV (genome): X. Sastre

- Yeast « Mutome » and « Meiotic recombination » (genome) : A. Nicolas

- RNAseq: Tumor Ewing (transcriptome): O. Delattre

- Long Non-Coding RNAs: A. Morillon

- ChIP-Seq: Mouse Spi1 transcription factor : C. Guillouf, F. Moreau-Gachelin

- Using NGS for genetic diagnosis : BRCA1, BRCA2, RB1 (C. Houdayer, HPV (X. Sastre)

- Evaluation of AmpliSeq Cancer Panel of Life Technologies

Quality Assurance /Certification:
- Medical diagnosis

For further information:ngs.curie.fr

Scientist in charge:
Alain Nicolas
Platform Manager:
Thomas Rio Frio

Institut Curie, 26 rue d’Ulm 75248 Paris Cedex 05

Alain Nicolas
01 56 24 65 20

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