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Sequencing Platform Genomic Paris Centre - Institut Curie

Expertise
- Targeted resequencing and whole genome sequencing, ChIP-seq, RNA-seq, SmallRNA.
- Fragment libraries, paired-end and mate pair libraries
- Amplicon sequencing, target enrichment, exome and whole genome sequencing
- Identification of polymorphisms, mutations and genome rearrangements..

Equipment
2 MiSeq Illumina
1 HiSeq 2500 Illumina
1 Sequel Pac Bio
1 Chromium 10X Genomics
1 C1 Single-cell auto Prep system (Fluidigm)
2 x Ion Torrent PGM (10Mb-1Gb)
Ion Proton (10Gb)

Main achievements
- BRCA1 and BRCA2 (targeted) : C. Houdayer, D. Stoppa-Lyonnet
- BRCAX (exome) :M-H. Stern, D. Stoppa-Lyonnet
- HPV (genome): X. Sastre
- Yeast « Mutome » and « Meiotic recombination » (genome) : A. Nicolas
- RNAseq: Tumor Ewing (transcriptome): O. Delattre
- Long Non-Coding RNAs: A. Morillon
- ChIP-Seq: Mouse Spi1 transcription factor : C. Guillouf, F. Moreau-Gachelin
- Using NGS for genetic diagnosis : BRCA1, BRCA2, RB1 (C. Houdayer, HPV (X. Sastre)
- Evaluation of AmpliSeq Cancer Panel of Life Technologies

Labels
- IBISA
- Medical diagnosis

Platform Managment
Scientist in charge:Alain Nicolas
Platform Manager: Sylvain Baulande

Institut Curie, 26 rue d’Ulm 75248 Paris Cedex 05

Contacts
Alain Nicolas
Alain.Nicolas@curie.fr
01 56 24 65 20

Sylvain Baulande
Sylvain.Baulande@curie.fr
01 56 24 67 38

For further information
ngs.curie.fr

last update : 2017


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