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"Paired-end" sequencing

The library preparation for "paired-end" sequencing, according to the lllumina technology, consists in fragmenting the genomic DNA mechanically (Covaris, Bioruptor) or enzymatically (tagmentase) to sizes below 1 kb.

Indexed and complementary adapters of the sequencing primers are then ligated at each end of the DNA fragments to ensure sequencing from both ends (Figure 1).

The technology using tagmentase, a modified transposase, fragments DNA and simultaneously adds sequencing adapters (Figure 2: Nextera Technology).

A bead size selection step allows producing libraries of specific average insert size, which may be necessary for assembling particular genomic regions.

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