Transcriptome regulation is a fundamental process of living cells ; it controls differentiation, specific gene expression, development and proliferation, and even environmental adaptation. The study of transcriptome regulation includes identification of elements of the genome that are involved in different biological processes. Methodological approaches such as ChIP-Seq, DNase-Seq and FAIRE-Seq allow analysis of regulatory regions of gene expression by identification of DNA regions associated with regulatory activities
Chromatin Immunoprecipitation Sequencing is a method used to analyse interactions between proteins and DNA. This application combines chromatin immunoprecipitation (ChIP), using specific antibodies for a protein of interest, and high throughput sequencing.
The goal is to map all DNA binding sites of this protein across the whole genome. Chromatin is composed of histone-type proteins and DNA.
Il est ainsi possible de cartographier toutes les régions génomiques associées avec une histone nucléosomale portant une modification biochimique (acétylation, méthylation, etc.) caractéristique de l’activité transcriptionnelle.
The principle consists of covalently bonding the proteins linked with the DNA using a chemical process, then fragmenting the chromatin, immunoprecipitating the fragments with a specific antibody and, after purification and elimination of the proteins, sequencing the DNA fragments obtained.
The France Génomique platforms in Marseille (TGML) and Paris Centre at the ENS site will accept requests for ChIP sequencing.
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