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Methylated DNA Immunoprecipitation (MeDIP)

DNA methylation is a phenomenon implicated in numerous diseases, the most frequently studied of which is cancer. In mammals, methylation occurs at cytosine bases which are followed by a guanine base (CpG), principally in the form of 5-methylcytosine :

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MeDIP is a technique that uses anti-methylcytosine antibodies which are coupled to magnetic beads to immunoprecipitate the regions that are sufficiently methylated.

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Sample DNA is fragmented by sonication then antibodies are added. Once these have bonded to the methylcytosines, the methylated fragments are collected using a magnet, thus removing from the sample any unmethylated or weakly methylated fragments : the sample has thus been enriched with methylated sequences and is ready to be analysed using a sequencing technique. For better reproducibility, it is recommended to use a robot to perform MeDIP.
The French National Research Centre for Human Genomics (CNRGH), which is part of the F Jacob Institute of Biology at the CEA in Evry (near Paris), and the Toulouse platform, both have the necessary equipment to optimise this pre-sequencing step.

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