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Bisuphite Conversion of DNA

In order to transform ADN methylation, a difficult to quantify epigenetic trait, into a genetic variation that is more easily detectable, bisulphite treatment is applied to convert the unmethylated cytosines into uraciles :

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Bisulphite conversion produces two distinct, non-complementary DNA strands.

Bisulphite conversion produces two distinct, non-complementary DNA strands.
Following PCR amplification of one of these strands, the unmethylated cytosines are “replaced” by thyamines. The percentage of cytosines remaining represents the percentage of methylation at a given position.

The CNRGH in Evry (near Paris), the Toulouse and Curie platforms have the necessary know-how for optimal performance of this technique.


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